abstract：:We compared cardiac troponin I (cTnI), using Access, Sanofi Pasteur, and cardiac troponin T (cTnT), using Elecsys, Boehringer Mannheim, in the first two routine blood samplings in a routine panel of cardiac markers for the biochemical diagnostic evaluation of patients with symptoms of acute myocardial infarction (AMI)...

journal_title：Clinical chemistry

authors： Hetland O,Dickstein K

更新日期：1998-07-01 00:00:00

abstract：:We describe a rapid and simple insulin RIA in which proinsulin and conversion intermediates do not interfere. Three monoclonal antibodies (S1, S2, and S53) were selected for their specificity (directed, respectively, against the B10 region, the junction between A chain and C-peptide, and the junction between B chain a...

journal_title：Clinical chemistry

authors： Deberg M,Houssa P,Frank BH,Sodoyez-Goffaux F,Sodoyez JC

更新日期：1998-07-01 00:00:00

abstract：:Urokinase plasminogen activator (uPA) is a serine protease causally involved in cancer invasion and metastasis. In this study, high concentrations of uPA in primary breast cancers were independently associated with both a shortened disease-free interval and overall survival. For the disease-free interval as endpoint, ...

journal_title：Clinical chemistry

authors： Duffy MJ,Duggan C,Mulcahy HE,McDermott EW,O'Higgins NJ

更新日期：1998-06-01 00:00:00

abstract：:The herbicide paraquat (1,1'-dimethyl-4,4'-bipyridylium dichloride; PQ), is a poison known to cause delayed mortality due to lung and kidney injuries. High-resolution proton nuclear magnetic resonance (1H NMR) spectroscopy has been extensively applied in evaluating nephrotoxicity by the characteristic perturbations in...

journal_title：Clinical chemistry

authors： Bairaktari E,Katopodis K,Siamopoulos KC,Tsolas O

更新日期：1998-06-01 00:00:00

abstract：:The effect of serum-clot contact time on laboratory results was studied by dividing each blood specimen into four blood collection tubes. The control sera were separated from the clot within 30 min of the collection. The other tubes were incubated at 32 degrees C, and the sera were separated at 3, 6, and 24 h. The ser...

journal_title：Clinical chemistry

authors： Zhang DJ,Elswick RK,Miller WG,Bailey JL

更新日期：1998-06-01 00:00:00

abstract：:The poly-T tract in intron 8 of the cystic fibrosis conductance transmembrane regulator (CFTR) gene exists in three variants, 5T, 7T, and 9T. The 7T and 9T variants generate a predominantly normal transcript, whereas the 5T variant engenders an anomalous product. The analysis of the poly-T tract is assuming increasing...

journal_title：Clinical chemistry

authors： Gelfi C,Perego M,Righetti PG,Cainarca S,Firpo S,Ferrari M,Cremonesi L

更新日期：1998-05-01 00:00:00

abstract：:The use of 4-chlorotestosterone acetate by farmers for cattle fattening was recently demonstrated although the use of this anabolic steroid is strictly forbidden in the European Union. We investigated the metabolism of 4-chlorotestosterone acetate in the bovine species after intramuscular and oral administration. Nine...

journal_title：Clinical chemistry

authors： Le Bizec B,Montrade MP,Monteau F,Gaudin I,Andre F

更新日期：1998-05-01 00:00:00

abstract：:The serum pancreolauryl test has limited sensitivity for detecting mild pancreatic insufficiency. The aim of this study was to optimize the serum pancreolauryl test so as to increase the probability of positive results in patients with chronic pancreatitis. The study had three parts. First, the sampling time was optim...

journal_title：Clinical chemistry

authors： Domínguez-Muñoz JE,Malfertheiner P

更新日期：1998-04-01 00:00:00

abstract：:We report a survey on urine drug testing within a total of 269 laboratories of the European Union. Clinical laboratories predominated over forensic laboratories (59.5% vs 28.5%). Screening without identification/quantification was the common approach used by clinical laboratories, whereas screening with identification...

journal_title：Clinical chemistry

authors： Badia R,de la Torre R,Corcione S,Segura J

更新日期：1998-04-01 00:00:00

abstract：:The most popular pretreatment method of plasma samples for the measurement of ascorbate (AsA) and dehydroascorbate (DHA) has been an acidic deproteinization via metaphosphoric acid or trichloroacetic acid. In general, DHA is absent in plasma samples prepared from human blood in a conventional manner. However, when the...

journal_title：Clinical chemistry

authors： Koshiishi I,Mamura Y,Liu J,Imanari T

更新日期：1998-04-01 00:00:00

abstract：:Circulating prostate cells can be detected in cancer patients by using reverse transcriptase-PCR (RT-PCR) assay for prostate-specific antigen (PSA) and prostate-specific membrane antigen (PSM) mRNA. A quality-control study involving a conventional RT-PCR assay was performed and, surprisingly, detected both transcripts...

journal_title：Clinical chemistry

authors： Gala JL,Heusterspreute M,Loric S,Hanon F,Tombal B,Van Cangh P,De Nayer P,Philippe M

更新日期：1998-03-01 00:00:00

abstract：:6-thioguanine (6-TGN) and methyl 6-mercaptopurine nucleotides (Me6-MPNs) are the two major metabolites found in erythrocytes after administration of azathioprine. In an attempt to understand the role of these metabolites in the pharmacologic and toxic activity of thiopurines, we have developed a HPLC method for the si...

journal_title：Clinical chemistry

authors： Dervieux T,Boulieu R

更新日期：1998-03-01 00:00:00

abstract：:A region consisting of 19 clinical laboratories harmonized their calibration of seven common enzymes by using fresh patient-pool sera. One of the laboratories was chosen to act as Regional Reference Laboratory (RRL). This laboratory used internationally accepted (mostly IFCC) methods at 37 degrees C, with an intralabo...

journal_title：Clinical chemistry

authors： Franck PF,Steen G,Lombarts AJ,Souverijn JH,van Wermeskerken RK

更新日期：1998-03-01 00:00:00

abstract：:Advanced glycosylation end products (AGEs) are thought to play an important role in the development of diabetic complications. Oxidative reactions are essential for the formation of some AGEs, termed glycoxidation products. Increased concentrations of pentosidine, one of such products, are found in tissue and serum in...

journal_title：Clinical chemistry

authors： Rodríguez-García J,Requena JR,Rodríguez-Segade S

更新日期：1998-02-01 00:00:00

abstract：:A rapid and precise immunoassay for quantification of total homocysteine in blood samples is presented. The method avoids the use of radioisotopes and chromatographic separations and relies on enzymatic conversion of homocysteine to S-adenosyl-L-homocysteine, followed by quantification of S-adenosyl-L-homocysteine by ...

journal_title：Clinical chemistry

authors： Frantzen F,Faaren AL,Alfheim I,Nordhei AK

更新日期：1998-02-01 00:00:00

abstract：:Several factors can limit the use of therapeutic drug monitoring (TDM) for cancer chemotherapeutic agents, including poorly defined concentration-effect relationships for many antineoplastic agents. This is further complicated by cancer being a highly heterogeneous group of diseases, each of which may have a unique co...

journal_title：Clinical chemistry

authors： Hon YY,Evans WE

更新日期：1998-02-01 00:00:00

abstract：:Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency is a recessively inherited defect in the mitochondrial beta-oxidation of fatty acids. A single nucleotide change, the A985 --> G transition, in the MCAD gene accounts for approximately 90% of all the disease-causing mutations in the patients. We have used PCR to am...

journal_title：Clinical chemistry

authors： Romppanen EL,Mononen T,Mononen I

更新日期：1998-01-01 00:00:00

abstract：:The values of apolipoproteins (apo) A-I and B were determined in a population sample of hospital outpatients with a standardized method to verify if the cutpoints calculated in a cross-sectional study in the US are usable with other populations. We also tested the apolipoproteins' ability to discriminate between healt...

journal_title：Clinical chemistry

authors： Graziani MS,Zanolla L,Righetti G,Marchetti C,Mocarelli P,Marcovina SM

更新日期：1998-01-01 00:00:00

abstract：:Single-strand conformation polymorphism (SSCP) analysis is widely used to prescreen mutations in p53 gene. However, standardization of SSCP to detect p53 mutations has rarely been pursued so far. We have developed complete conditions for a temperature-controlled nonradioactive SSCP for mutation detection in amplified ...

journal_title：Clinical chemistry

authors： Welsh JA,Castrén K,Vähäkangas KH

更新日期：1997-12-01 00:00:00

abstract：:Four double-monoclonal time-resolved immunofluorometric assays (TrIFMAs) have been developed for the specific determination of pregnancy-associated plasma protein A/proeosinophil major basic protein (PAPP-A/ proMBP) complex in first-trimester maternal serum samples. The assays have a functional sensitivity of < 4 mIU/...

journal_title：Clinical chemistry

authors： Qin QP,Christiansen M,Oxvig C,Pettersson K,Sottrup-Jensen L,Koch C,Nørgaard-Pedersen B

更新日期：1997-12-01 00:00:00

abstract：:Reference values were determined for 23 plasma free amino acids from measurements done in 148 healthy children ranging from 0 to 18 years of age. Amino acid analysis was performed by ion-exchange chromatography. We propose a graphic form of presenting the age-specific distribution of plasma amino acid concentrations w...

journal_title：Clinical chemistry

authors： Lepage N,McDonald N,Dallaire L,Lambert M

更新日期：1997-12-01 00:00:00

abstract：:We have assessed the technical performance and robustness of NycoCard CRP Whole Blood, a near-patient test for C-reactive protein (CRP), when used in realistic daily routine situations in general practice clinics (GPC). Thirteen GPCs participated, five of them with technician staff. From 898 patients, split-sample mea...

journal_title：Clinical chemistry

authors： Dahler-Eriksen BS,Lassen JF,Petersen PH,Lund ED,Lauritzen T,Brandslund I

更新日期：1997-11-01 00:00:00

abstract：:The diagnosis of thyroid disease now often can be achieved reliably by measuring thyrotropin (TSH) alone. Thyroxine (T4), triiodothyronine, and other analytes are only needed if TSH and the accompanying clinical condition are discordant. We describe here work that confirms the age independence of TSH in both inpatient...

journal_title：Clinical chemistry

authors： Davey R

更新日期：1997-11-01 00:00:00

abstract：:The formal definition of sensitivity associates the term with the change in the response of a system for a small change of the stimulus causing the response, i.e., the ratio of the response of a system to the stimulus causing it. One interpretation of sensitivity associates the rate of change of the response for a sma...

journal_title：Clinical chemistry

authors： Pardue HL

更新日期：1997-10-01 00:00:00

abstract：:We have developed a liquid chromatography-isotope dilution mass spectrometry procedure to quantify total cholesterol in serum. A particle-beam interface was used for coupling the liquid chromatograph and the mass spectrometer. After electron impact ionization the ions m/z = 386 and m/z = 389 were used for selective io...

journal_title：Clinical chemistry

authors： Kock R,Delvoux B,Greiling H

更新日期：1997-10-01 00:00:00

abstract：:Development of a new, sensitive immunoassay for measuring transforming growth factor beta 1 (TGF-beta1) is described and compared with four commercially available TGF-beta1 immunoassays. Preanalytical conditions were evaluated. The nonlinearity found in serum or plasma is due to masking of TGF-beta1 by binding protein...

journal_title：Clinical chemistry

authors： Kropf J,Schurek JO,Wollner A,Gressner AM

更新日期：1997-10-01 00:00:00

abstract：:We evaluated the analytical performance of new estradiol and progesterone assays performed on the Bayer Immuno 1 system. Within-run and between-day CVs for estradiol at concentrations of 116.8-6645.8 pmol/L were < or = 6.4% and for 5.54-103.95 nmol/L progesterone were < or = 7.7%, thus meeting published analytical goa...

journal_title：Clinical chemistry

authors： Lévesque A,Letellier M,Dillon PW,Grant A

更新日期：1997-09-01 00:00:00

abstract：:Five genetic defects have been established as risk factors for venous thrombosis. Three are protein C, protein S, and antithrombin deficiencies, defects in the anticoagulant pathways of blood coagulation. Together they can be found in approximately 15% of families with inherited thrombophilia. Their laboratory diagnos...

journal_title：Clinical chemistry

authors： Bertina RM

更新日期：1997-09-01 00:00:00

abstract：:Red blood cell (RBC) replacement solutions are being developed as alternatives to allogeneic RBC use in blood transfusions in the treatment of massive trauma, to achieve hemodynamic stability during elective surgery, and to increase oxygen-carrying capacity in anemia. Hemoglobin-based oxygen carrier (HBOC)-201 (Biopur...

journal_title：Clinical chemistry

authors： Callas DD,Clark TL,Moreira PL,Lansden C,Gawryl MS,Kahn S,Bermes EW Jr

更新日期：1997-09-01 00:00:00

abstract：:Hepatitis A virus (HAV) infection occurs worldwide and is an important cause of acute viral hepatitis in the US. In this review, I cover the epidemiology, course of infection, clinical manifestations, serological responses, and prevention of this infection. Although most patients completely recover from this disease, ...

journal_title：Clinical chemistry

authors： Lemon SM

更新日期：1997-08-01 00:00:00

abstract：:We combined the amplification refractory mutation system (ARMS) and fluorescence polarization (FP) to give a homogeneous genomic DNA genotype analysis method. Oligonucleotide probes labeled with the fluorescein dyes fluorescein isothiocyanate and 5-([4,6-dichlorotriazin-2-yl]amino)fluorescein and the rhodamine dye 6-c...

journal_title：Clinical chemistry

authors： Gibson NJ,Gillard HL,Whitcombe D,Ferrie RM,Newton CR,Little S

更新日期：1997-08-01 00:00:00

abstract：:We have developed a new assay for cortisone (E) in serum, saliva, and urine involving Celite chromatography followed by RIA with 125I-labeled E and scintillation proximity assay. The chromatography step separates cortisol (F) from E, and in combination with their RIAs, permits assessment of the status of the F-E shutt...

journal_title：Clinical chemistry

authors： Morineau G,Boudi A,Barka A,Gourmelen M,Degeilh F,Hardy N,al-Halnak A,Soliman H,Gosling JP,Julien R,Brerault JL,Boudou P,Aubert P,Villette JM,Pruna A,Galons H,Fiet J

更新日期：1997-08-01 00:00:00

abstract：:A new method for the reliable identification of localized variations in DNA by detection of associated diagnostic products with matrix-assisted laser desorption ionization time-of-flight mass spectrometry is described. The diagnostic products are generated by the primer oligo base extension (PROBE) reaction, which req...

journal_title：Clinical chemistry

authors： Braun A,Little DP,Köster H

更新日期：1997-07-01 00:00:00

abstract：:The concentrations of 25 plasma proteins were measured in 22 patients with membranous nephropathy. For some large proteins, the plasma concentrations were increased; there were also large proteins with low plasma concentrations, but small or medium-sized proteins showed uniformly lower plasma concentration than the co...

journal_title：Clinical chemistry

authors： Joven J,Clivillé X,Camps J,Espinel E,Simó J,Vilella E,Oliver A

更新日期：1997-07-01 00:00:00

abstract：:The Arg-to-Trp substitution at codon 3500 in the apolipoprotein (apo) B-100 gene is established as a cause of familial defective apo B-100 (FDB), a functional mutation, resulting in reduced LDL receptor binding and manifest hypercholesterolemia. In a search for similar mutations in 163 Malaysians, we screened the puta...

journal_title：Clinical chemistry

authors： Choong ML,Koay ES,Khoo KL,Khaw MC,Sethi SK

更新日期：1997-06-01 00:00:00

abstract：:Carbohydrate-deficient transferrin (CDT) may now be the most valuable biological marker for diagnosis of alcohol abuse. We compared the diagnostic performance of two new CDT tests, Axis %CDT turbidimetric immunoassay (TIA) and Axis %CDT HPLC, against Specialty Laboratories' isoelectric focusing/immunoblotting/laser de...

journal_title：Clinical chemistry

authors： Bean P,Liegmann K,Løvli T,Westby C,Sundrehagen E

更新日期：1997-06-01 00:00:00

abstract：:We evaluated the performance of two homogeneous assays for quantifying HDL cholesterol (HDL-C) and compared them with the phosphotungstic acid (PTA)/ MgCl2 assay. Both homogeneous HDL-C assays were precise, having a within-run CV of < 1.20% and a between-run CV of < 4.07%. The HDL-C values (y) measured by the two homo...

journal_title：Clinical chemistry

authors： Huang YC,Kao JT,Tsai KS

更新日期：1997-06-01 00:00:00

abstract：:We review the present knowledge of telomeres and telomerase with special attention to their role in cell proliferation, cellular senescence, and human aging. We summarize the functional aspects of telomerase in cancer, as well as its role as a useful diagnostic and prognostic tumor marker, and discuss possible approac...

journal_title：Clinical chemistry

authors： Dahse R,Fiedler W,Ernst G

更新日期：1997-05-01 00:00:00

abstract：:An electrospray ionization-mass spectrometric procedure has been developed for determining glycohemoglobin. Whole-blood samples from 78 diabetic and 50 nondiabetic subjects (glycation range 3-15%, as determined by electrospray mass spectrometry) were diluted 500-fold in an acidic denaturing solvent and introduced dire...

journal_title：Clinical chemistry

authors： Roberts NB,Green BN,Morris M

更新日期：1997-05-01 00:00:00

abstract：:The topic of quality control often evokes prolific discussion among those whose business is its administration. Reliable methods for identifying mistakes must be developed in both the manufacture and use of test devices. Unit test devices are uncomplicated to run and provide quick results in a world where speed and si...

journal_title：Clinical chemistry

authors： Blase AB

更新日期：1997-05-01 00:00:00